The FOXP3 antibody is a 431 amino acid protein and is also a member of the forkhead/winged-helix family. It is essential for regular immune homeostasis. It is constitutively expressed at a higher level in CD35 and CD4 positive regulatory T-cells, as well as lower levels of CD4 positive and CD25 negative cells. It is absent in CD4 negative and CD8 positive t-cells. It is considered to be a master regulatory gene and a specific marker for regulatory T-cells.
It should not be used for diagnostic procedures and is designed to be used for research purposes. There is no available clone, and the immunogen is the synthetic peptide derived from the human c-terminus FOXP3 protein. The isotype is the Rabbit IgG with an undetermined epitope. The molecular weight of this antibody is 50kDa.
It is designed for use with Immunohistochemistry applications. To prepare your specimen, use a Formalin-fixed or paraffin-embedded tissue that is suitable. Deparaffinized slides are required, and you can clean them with graded alcohols or xylene.
You can find a pre-diluted and concentrated version of the FOXP3 antibody. When choosing the concentrated format, you will need to dilute the antibody using a ratio of 1:200. Using the pre-diluted form can remove one step from the procedure.
Antigen retrieval is done by boiling tissue sections in a 1mM EDTA with a pH of 8.0 for 10 minutes. Allow it to cool completely at room temperature for 20 minutes. Once it has cooled for the correct time period, incubate for 30 minutes while at room temperature.
The positive control of this antibody is the tonsil with a cellular localization in the nucleus.
The FOXP3 antibody is designed to help you test for a variety of situations in the T-cells. Visit Spring Bioscience to learn more and find purchase options.