Laboratory Equipment

Common Mistakes When Using A Genetic Sequencer

With many of the new designs and options in DNA sequencers from top manufacturers such as Applied Biosystems and Illumina, the demands on technicians are reduced. This means that the testing is fully automated, eliminating human error that can lead to many problems during the process. However, even with the top genetic sequencer, there are still some important issues to consider to ensure the quality of results. In general, there are two common problems with any test results, and they include the incorrect use of the primer as well as using the best quality in DNA as the template. Quality Template Issues As with any type of testing situation, the end results are a direct correlation to the original material. When the genetic template or the original DNA is compromised, not collected using the appropriate protocols or when the incorrect prep kit is used, the genetic sequencer is already at a disadvantage. It is essential to follow all collection protocols and the specific miniprep and extraction methods. Always use new columns and never recycle to avoid any type of contamination. Primer Considerations While the genetic sequencer will handle the process based on the settings the technician inputs, the incorrect choice of primer is something that the tech needs to be careful to avoid. Primers should always be checked and analyzed before use and meet the correct characteristics for the specific sequencing requirements. Simple issues can also occur that can be devastating to the results. For example, failure to dilute the template DNA correctly or to use the wrong concentration or volume in the sequencer will always result in incomplete and inaccurate results. Taking the time to double check all aspects of the system before starting the sequencing can help to catch errors and prevent the need to repeat the...

Read More

Simple Checks When Buying Refurbished Medical Equipment

As a buyer of refurbished medical equipment, there are a few considerations to help ensure the best possible price. In addition, it will also be important to choose refurbished equipment that is fully restored to OEM (Original Equipment Manufacturer) specifications. Unfortunately, not all refurbished medical equipment is sold at a fair price. It is also not always refurbished; it may simply have been repaired. Understanding the differences will be essential for first-time buyers and until you find a reputable refurbished medical equipment company to partner with for future purchases. Years in the Business One of the easiest ways to determine the reputation of the medical equipment supplier you are considering is to find out how long they have been in business. Quality businesses will have years in the industry, with some in business for decades. In this industry, customer service, pricing and quality of equipment is essential. Simply staying in business is a testimony to the care taken during the refurbishing process and the commitment to customer support and fairness in pricing. Refurbished, Repaired or Used While the words refurbished, repaired or used may seem the same, they are actually very different. Repaired equipment typically means that any damaged parts are replaced and the equipment is functioning. However, it is typically not upgraded nor are the repair parts necessarily from the original manufacturer. On the other hand, refurbished medical equipment uses only OEM parts. For applicable devices, it typically includes software upgrades. The best companies will provide this equipment with a warranty, typically six months or more, providing assurance to the buyer. Used equipment is typically sold “as is” without any repairs or refurbishing. It will be cleaned and sanitized, but this is also important to verify. Generally, used equipment will be tested but will not have a warranty, but there can be exceptions so be sure to...

Read More


Pin It on Pinterest

Copyright Base Articles © 2011 - 2017. All Rights Reserved